Basic and Applied Aspects of Seed Biology: Proceedings of by F. A. Hoekstra, W. F. Wolkers, J. Buitink, E. A. Golovina

By F. A. Hoekstra, W. F. Wolkers, J. Buitink, E. A. Golovina (auth.), R. H. Ellis, M. Black, A. J. Murdoch, T. D. Hong (eds.)

The 5th overseas Workshop on Seeds was once held on the collage of examining, united kingdom, from 10 to fifteen September, 1995. a few 230 seed scientists, from a variety of disciplines (botanists, biochemists, ecologists, agriculturalists, foresters, and advertisement seedsmen), from 31 nations (Europe, the Americas, and Asia) participated within the workshop. quite a few oral and poster displays was once made throughout the workshop and we're happy to submit such a lot of of them in those court cases. The papers herein are indexed through the periods within which they have been awarded yet, as is frequently the case, many papers disguise a broader variety of issues than the consultation titles suggest. For seed physiologists, ecologists, and technologists, this booklet collates a lot of the present examine on seeds.

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Carpenter, lE and Crowe, lH. 1989. Biochemistry 28: 3916-3922. M. and Crowe, lH. 1987a. Biochimica et Biophysica Acta 923: 109-115. M. H. 1987b. Cryobiology 24: 455--464. Crocker,W. and Groves, lE 1915. Proceedingsofthe NationalAcademyofSciences U. A. 1: 152-155. Crowe, lH. M. 1992. In: Liposome Technology, 2nd Edition, (ed. G. Gregoriadis). Boca Raton, FL: CRC Press. , Carpenter, IF. and Aurell Wistrom, C. 1987. Biochemical Journal 242: 1-10. l and Hoekstra, FA. 1995. In: Handbook ofPhysiology, (in press) (ed.

1994b). Neutral Lipid Quantitation Embryo samples (50-80 mg) were suspended in·CHCh (10 mI, room temperature), shaken for 5 min and filtered. The process was repeated a second time in the same volume of CHC13 for 30 min. The two CHCh extracts were pooled and evaporated under reduced pressure at 40°e. In order to verify that the only lipids present in the extracts were the neutral ones, the extracts were subjected to TLC analysis. TLC silica gel plates were developed in CHC13 • Lipids were identified by using authentic samples as reference and by spraying with sulphocromic mixture followed by heating at 120°e.

After humid air treatment this pollen can germinate, which implies that the fore-mentioned gel phase is fully reversible. This is also true with regard to cold storage at -20°C, a condition that promotes the presence ofgel phase domains in the membranes of dry orthodox pollen and seed. As storage at -20°C can be endured without loss ofviability, this sort of gel phase is also reversible. It is not expected that these gel phase domains lead to extensive phase separations, since such events are much more disrupting and not entirely reversible.

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