Arrhythmias and sudden death in athletes by X. Wehrens, A. Marks

By X. Wehrens, A. Marks

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81 The more C-terminal 160 kDa fragment reacted with an antibody against an epitope contained within aa 4382-4417 and was resistant to extraction,81 implying that TM sequences exist in the sequence between aa 3119 and 4476. This sequence would include M’, M”, M1, M2, M3 and M4 from the Zorzato model. The 76 kDa C-terminal fragment (4476-5037) could not be extracted into indicating that it was membraneassociated81. This fragment would include M5 to M10 from the Zorzato model and M1 to M4 from the Takeshima model.

4-2). In addition it is clear that overall the DHPR tetrad is larger than the outline of the foot and this in part explains why tetrads are associated with alternate feet. These images define specific restrictions on the location of DHPRs, which will acquire importance in the near future, once higher resolution images will be available. 133 More on this below. 131 Structural observations give some clues to the possible role of RyR3. 138 Identification of these parajunctional feet with RyR3 (Fig.

The location of other mutation-sensitive aa in TM segments has been investigated in a number of experiment. In a study of the mutationsensitivity of conserved polar aa in TM sequences,75 mutations in in M2, and in M7 and and in M10 had functional consequences that suggested that they might be involved in channel function and regulation. 76 These results do not provide direct evidence, however, that the sequences in which these critical residues lie are, in fact, TM sequences. 79 These two groups of mutants are largely located on opposite sides of the M10 helix.

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