A Pet That Fits

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In A. parasiticus and A. flavus, the aflatoxin gene cluster consists of 24 genes or ORFs, within an approximately 70 kb region of DNA (Figure 1). In A. parasiticus, a partial duplicated aflatoxin gene cluster consisting of seven genes, aflR2, Meeting the mycotoxin menace 31 D. A. E. F. Robens aflJ2, adhA2, estA2, norA2, ver1B, omtB2, has been confirmed and characterized (Chang and Yu, 2002). There is a positive regulatory gene, aflR, located in the middle of the gene clusters for activating pathway gene transcription.

Genomic organization of the Fusarium trichothecene gene cluster. A. Relative scale in kilobases. B. Organization of the predicted ORFs for F. sporotrichioides and F. graminearum. The arrows heads indicate direction of transcription. g. 5 indicates TRI5). Genes from different Fusarium species with the same number are homologues. F. graminearum lack a functional TRI7 and TRI13 genes. C. Table listing all of the trichothecene genes, their predicted enzyme function and the carbon molecule affected on the trichothecene skeleton.

Council for Agricultural Science and Technology Task Force Report No. 139, (pp. 199). Ames, Iowa. , 2003. Secondary metabolic gene clusters in filamentous fungi. D. and Bhatnagar, D. (eds), Marcel Dekker (in press), New York, NY. -K. , 2002. Characterization of a partial duplication of the aflatoxin gene cluster in Aspergillus parasiticus ATCC 56775. Appl. Microbiol. Biotechnol. 58, 632-636. R. , 1999. A corn trypsin inhibitor with antifungal activity inhibits Aspergillus flavus α-amylase. Phytopathology.

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